Urinary Tract Infection (UTI) is an infection that affects part of the urinary tract. It accounts for nearly 25% of all infections. Recurrences usually occur within three months of the original infection. It is the most frequent bacterial infection in women which occurs four times more frequently in female than male. Changing epidemiology of infectious disease with the advent of antimicrobial agents, now we realize that as we developed antimicrobial agents, microbes developed the ability to elude our best weapons and to counterattack with new survival strategies. Antibiotic resistance occurs at an alarming rate among all classes of mammalian pathogens. If we observe and evaluate the quotation mentioned in Ayurveda, we will come to know that the origins of modern microbiology are deeply situated in ancient literature. It was decided to evaluate this knowledge with the help of present day technique such as urine culture and sensitivity method by using extract derived from seeds and flowers of Butea Monosperma.